Fig. S4.

Characterization of the Tet1 distal transcriptional enhancer (Fig. 4). (Top) The region upstream of Tet1 is shown in the UCSC genome browser, with the core of CpGI 1 marked in green and the CAGE data for transcript initiation sites. The region transcribed to eRNA is shown together with the locations of various primers used for its amplification (primers marked in red were successful and those in black were not). (Bottom) The region upstream of the Tet1 functional TSS in the gonadotropes is marked, with vertical arrows signifying Dpn2 restriction sites and horizontal arrows the location of primers used in the 3C assay. The numbers in red refer to the primer identity. “Ex 1” refers to the canonical exon 1, and the CpGI is CpGI 1 at the region of the enhancer.