Table S1.
Primers
Primer no. | Gene | Sequence |
RT-PCR | ||
76 | GAPDH +51F | ATGGTGAAGGTCGGTGTGAA |
77 | GAPDH +280R | TCCTGGAAGATGGTGATGGG |
350 | Lhb +179F | CTAGCATGGTCCGAGTACTG |
351 | Lhb +301R | CTGAGGGCTACAGGAAAGGA |
184 | RPLP0 +196F | GCGACCTGGAAGTCCAACTA |
185 | RPLP0 +296R | ATCTGCTTGGAGCCCACAT |
326 | Tet1 exon 11F | GAGCCTGTTCCTCGATGTGG |
327 | Tet1 exon 11R | CAAACCCACCTGAGGCTGTT |
790 | Tet1 exon 1F | TCCTAGGACAGGCCTTTAGT |
791 | Tet1 exon 1R | CTATCCATATCCATAGCCAC |
792 | Tet1 exon 3F | CTGGAACAAGTGGTAGCCAT |
793 | Tet1(5′RACE) exon 3R | GGTCTTTTCTCACCGAATGA |
798 | Tet1 exon 2F | GTTACAAAAGAAAACAAGAGGC |
800 | Tet1 exon 1.5F | GGAGGCTTCCTCTGCAGAA |
801 | Tet1 exon 1.5R | TGCATAAATAAAGATGGAGGG |
804 | Tet1 exon 2R | CTTTAAAACTTTGGGCTTCTTT |
799 | Tet1(5′RACE nested) exon 3R | TTCAAGGGGATGTGTGATCAA |
292 | Tet2 +4248F | ACTTCTCTGCTCATTCCCACAGA |
293 | Tet2 +4347R | TTAGCTCCGACTTCTCGATTGTC |
294 | Tet3 +3393F | GAGCACGCCAGAGAAGATCAA |
295 | Tet3 +3492R | CAGGCTTTGCTGGGACAATC |
219 | Fshb +637F | GCTGGAGAGCAATCTGCTGCCA |
220 | Fshb +795R | TATTGGGCCGAGCTGGGTCCTTA |
35 | Cga +97F | ATGGATTACTACAGAAAATATGCAG |
36 | Cga +196R | CCTGAATAATAAAGTCTCCATCAGG |
1194 | PGR F | CAGAAAGGGGTTGTCCCCAG |
1195 | PGR R | TTCCGGAAATTCCACAGCCA |
ChIP/MeDIP/5hmC real-time PCR | ||
21 | Lhb +591F | GTCCGAGTACTGCCGGCT |
351 | Lhb +727R | CTGAGGGCTACAGGAAAGGA |
661 | Lhb −107F | CCCAAAGAGATTAGTGTGTCTAG |
662 | Lhb −33R | TGCGGGTTGTGGGGGTGGCA |
363 | Lhb −83R | GCTTGGGTAACCTAGACACT |
260 | Lhb −284F | GACGTTTACTCCAGCAATCTGG |
181 | Lhb −151R | GTGTCCCAGTGAATTGGCCTCA |
370 | Lhb −170F | CACTGGGACACTGGAGCTA |
366 | Lhb −518F | CTTACTTCCAGAGTTCCTCC |
367 | Lhb −401R | GCACCACCGTCTCCCAGCCC |
55 | Lhb −950F | ATCTGAACTCTGCTGCACTT |
56 | Lhb −700R | TGTACTGGGCAAATTCGGTC |
59 | Lhb −1900F | GAATCTTTGGGCTCCCTTC |
60 | Lhb −1700R | CAGGCTCCTTCCTGCAGTGC |
727 | Lhb −4849R | GGCAGTTCCCAAGCCTGGA |
596 | Lhb −5005F | GCTCTCAGAATGCAAGGCTA |
396 | Lamc3 +89F | ACATGGGCTCTTGCTACGAC |
397 | Lamc3 +316R | GGCTGTGGAAGTCTGTGAGG |
415 | GAPDH −226F | GGAAGCAGCATTCAGGTCTC |
416 | GAPDH −148R | CAGGATAGGACTCAGGGAATACAG |
808 | Tet1 −24,439F | GTAAGTACACCATAGCTGTCCT |
809 | Tet1 −24,314R | AGTGCCCACTGCTCTTCCTAA |
831 | Tet1 −22,274F | GTCACCTGGATGACCCGACT |
1154 | Tet1 −22,098F | AGGAAGGAGTGGACAGGAGAA |
1155 | Tet1 −22,037F | TCGCATTTTGCCTCTCGCTCA |
626 | Tet1 −21,911F | GTACCCGCGCACTCCGCTC |
1168 | Tet1 −21,800F | CTGCCTCTTCTACGGGAACATTCG |
627 | Tet1 −21,782R | GTTCCCGTAGAAGAGGCAGG |
628 | Tet1 −21,491F | GGATCTCGATCGGGCTAGG |
830 | Tet1 −21,420R | GCGCGGAGTTGACTCGCAC |
629 | Tet1 −21,361R | CGCGAGCATTGTTAGTCACC |
785 | Tet1 −21,187F | GGCGATAGCTTTTGCCAGCGA |
783 | Tet1 −20,943F | GACTGGTTCTGGGATCCTGGA |
784 | Tet1 −20,840R | GCTCCCGGAGCTCACGTGCA |
787 | Tet1 −19,349F | GAGTTAACTATTGCTCTCAGATT |
788 | Tet1 −19,216R | CAGGAAGGATGTGTCCAGAAG |
643 | Tet1 −14,665F | GGCAACACCTCCAGATTCTC |
770 | Tet1 −14,572R | CTCGAGGGCTGCAGAGTAAGTAAAGA |
843 | Tet1 −191F | GCTAAGGGAGACTCCTCCAT |
853 | Tet1 −2R | AAGCTTTCCCAGACAAAGGATCTCCT |
839 | Tet1 −53F | GCCTGTGTCCTGTGGTGAGA |
838 | Tet1 +68R | CCACAAAGAGTGCAGCCAAT |
1178 | Tet1 (−1900) F | TTGCTCTATACAGCTATGGCT |
1179 | Tet1 (−1600) R | CTGCCCTGCCCTGTACAAA |
1180 | Tet1 (−1580) F | TGGAGGATGGTGTAAAGAGAA |
1181 | Tet1 (−1300) R | TTGGCAGGATTGCCTGGCTGC |
1182 | Tet1 (−1280) F | ATCCTGCAAATGTACTGCAG |
1183 | Tet1 (−1000) R | ACTGCACCCATACTCAGTGA |
1184 | Tet1 (−950) F | TCAGGTGTGTGTGTGTGTGT |
1185 | Tet1 (−700) R | GATGACCTTCAGTGCCCAG |
1249 | Tet1 (−720) F | GTTTCTCTGGGCACTGAAGG |
1250 | Tet1 (−400) R | ATTGGCGATATTGAAGGCAG |
1189 | Tet1 (−330) F | GCACAATACCTTCCAGGGTT |
1190 | Tet1 (−1) R | TCCCAGACAAAGGATCTCCT |
173 | H19F (positive MeDIP control) | GCATGGTCCTCAAATTCTGCA |
174 | H19R (positive MeDIP control) | GCATCTGAACGCCCCAATTA |
624 | Tet1 -22,569F (negative MeDIP control) | GTGTAAAGCCACAAGCCAGT |
625 | Tet1 -22,464R (negative MeDIP control) | GCATATCCCAAGGTAAGATAG |
790 | Tet1 exon 1F (AR ChIP negative control) | TCCTAGGACAGGCCTTTAGT |
791 | Tet1 exon 1R(AR ChIP negative control) | CTATCCATATCCATAGCCAC |
1366 | Tubb3 ARE1 forward (AR ChIP positive control) | TGGCCCCCAGAACAGAAG |
1367 | Tubb3 ARE1 reverse (AR ChIP positive control) | TGGTGTTCCCACTCTGTACAATG |
BS conversion PCR | ||
1158 | Tet1 −296F | GTATAATATTTTTTAGGGTTGTTGTG |
1159 | Tet1-42R | CAAAACACAAACAACCACAAACTAC |
691 | Tet1 −21,929F | GGGTTGATTTGGTTGGGAGTATT |
692 | Tet1 −21,617R | CCTACACCAATCCTTAAACAAACT |
693 | Tet1 −21,639F | GTTTGTTTAAGGATTGGTGTAGGTTT |
694 | Tet1 −21,312R | ATCCTAACCAAAACTAACTAAACTT |
Chromatin conformation assay | ||
843 | Tet1 −191 | GCTAAGGGAGACTCCTCCAT |
842 | Tet1 −221 | GACCTGCCCTGGTGCCACC |
878 | Tet1 −3416 | GCATGTGCAGGACTGGAGAGA |
845 | Tet1 −14991 | GATTCACTATGTAGCTATGGATG |
844 | Tet1 −15011 | TGTAGCCCAGACTGGCCTCA |
873 | Tet1 −15122 | GAATCGCCATGTACATGCTGG |
874 | Tet1 −18282 | GGAACTTGCTCTGTAGACCAGG |
875 | Tet1 −19592 | GATATGCACCCTCCACTGCTG |
851 | Tet1 −21581 | CAGAGGCAGTTTAGGGTGAGC |
850 | Tet1 −21680 | GCCTCAGGCTCCAAAGTTGC |
852 | Tet1 −22485 | GCTATCTTACCTTGGGATATGC |
876 | Tet1 −24521 | GAGCCATTTCGCCTGCCCAA |