Fig. S3.

Relative deuterium uptake plots of caspase-6 maturation variants over the course of the H/D exchange experiment. The different colors represent the deuterium uptake profile of caspase-6 mutation variants FL C163S (black), D23A D179A (blue), D23A D179CT (green), ΔN D179CT (orange), and ΔN D179CT + VEID (red). The covered residues for each peptic peptide of caspase-6 mutation variants are indicated. The asterisk designates the D to A mutation in the proteolytic cleavage site in caspase-6. Error bars, SD of duplicate H/DX-MS measurements done on 2 separate days. The asterisk designates the D to A substitution at the indicated proteolytic cleavage site, rendering that site uncleavable.