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. 2017 Sep 5;114(38):10113–10118. doi: 10.1073/pnas.1705755114

Fig. 3.

Fig. 3.

Smad7 promotes pluripotency independent of canonical TGF-β/Smad signaling. (A) Smad7 promotes ESC self-renewal independent of TGF-β/BMP signaling. SFB–Smad7–tet-on cells were pretreated with 5 μM SB431542 (TGFBRi) or 10 μM Dorsomorphin (BMPRi) for 12 h and then cultured in indicated medium for another 3 d. qRT-PCR was used to analyze expression of indicated pluripotency markers. Data are shown as mean ± SEM; n = 3. (B) Inhibition of TGF-β/BMP signaling does not reverse the effect of siSmad7 in ESC pluripotency. CGR8 cells were transfected with 40 pM Smad7 siRNA or control siRNA and cultured with TGFBRi or BMPRi for 2 d. Cell lysates were subjected to Western blot analysis.