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Biochemical analysis of ABA-dependent AHG1 (closed circle) and ABI2 (open circle) regulation in the presence of RCAR3. The phosphatase activity of AHG1 (2 µM) and ABI2 (0.1 µM) set to 100% was assayed in the presence of twofold molar excess of RCAR3 protein and different ABA concentrations as indicated. AHG1 is poorly regulated by RCAR3 even at 10 µM ABA (n = 3; mean ± SD).
