Fig. 1.

Expression and purification of the LjLYS6 ectodomain. (A) Schematic representation of the LjLYS6 construct with residue boundaries indicated. (B and D) The size-exclusion chromatography profile of glycosylated (B) and deglycosylated (D) LjLYS6 ectodomain purified on a Superdex 200 10/300 GL column. In the size-exclusion chromatography profile, both glycosylated and deglycosylated LjLYS6 ectodomains elute as a single peak (P3 and P4 respectively). (C and E) Coomassie brilliant-stained SDS–PAGE gels of the corresponding size-exclusion chromatography fractions. Glycosylated and deglycosylated LjLYS6 ectodomain from P3 (C) and P4 (E) are visible as distinct bands ∼35 or 27 kDa, respectively (indicated by a black arrow).