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. 2017 Sep 5;114(38):E8118–E8127. doi: 10.1073/pnas.1706795114

Fig. 6.

Fig. 6.

Ljlys6, Mtlyk9, and Mtlyr4 are more susceptible to pathogens than the corresponding wild-type plants. (A) Transient expression of pro35:LjLys6:YFP and pro35S:MtLyk9 constructs in N. benthamiana leaves induces localized cell death. No cell death was observed on the mock-infiltrated leaves. (Scale bars: 1 cm.) (B, C, E, and F) Ljlys6-5 (B) and Mtlyr4-1, Mtlyr4-2, and Mtlyk9 (E) mutants are more susceptible to B. cinerea than the corresponding wild types and have significantly increased lesion sizes (C and F). (D and G) Quantification of the Botrytis genomic CutA gene relative to the Lotus ATP-synthase (D) and Medicago histone H2A (G) housekeeping genes. (H) CO8-induced priming of immunity is MtLyk9 and MtLyr4 dependent. Suppression of Pst DC3000 infection after CO8 pretreatment is impaired in Mtlyr4-1, Mtlyr4-2, and Mtlyk9 mutants. **P < 0.01 indicates significant differences (t test) in C; different letters over bars in F and H indicate significant differences (P < 0.01; t test).