Table 1.
Dissociation constants of LjLYS6 ectodomain and wheat germ agglutinin toward chitin oligomers
| Ligand | Kd, µM | SD, µM | n of replicates |
| LYS6 ectodomain | |||
| Maltodextrin | N.D. | — | 2 |
| Chitotetraose | N.D. | — | 3 |
| Chitopentaose | 277 | 118 | 3 |
| Chitohexaose | 71 | 38 | 4 |
| Chitoheptaose | 26 | 16 | 3 |
| Chitooctaose | 38 | 22 | 4 |
| Wheat germ agglutinin | |||
| Maltodextrin | N.D. | — | 2 |
| Chitotetraose | 7.6 | 1.4 | 3 |
| Chitopentaose | 1.7 | 0.3 | 3 |
| Chitohexaose | 2.2 | 0.4 | 3 |
| Chitooctaose | 1.5 | 0.8 | 4 |
Dissociation constants of the LjLYS6 ectodomain and wheat germ agglutinin with various ligands as measured on a Monolith NT.115 system. Replicates for the LYS6 ectodomain are biological replicates; replicates for wheat germ agglutinin are technical replicates. Maltodextrin was used as a negative control. N.D., not detectable.