Skip to main content

View full-text article in PMC

. 2017 Sep 5;114(38):10101–10106. doi: 10.1073/pnas.1706978114

Table 1.

Initial kinetic parameters

	HsPRMT5/MEP50				HsPRMT1
Substrate	K_0.5, μM	k_cat, h⁻¹	k_cat/K_0.5, h⁻¹·μM⁻¹	n (Hill coefficient)	K_0.5, μM	k_cat, h⁻¹	k_cat/K_0.5, h⁻¹·μM⁻¹	n (Hill coefficient)
H4 (1-21) WT^*	0.39 ± 0.033	5.63 ± 0.36	14.44	2.83 ± 0.66	0.58 ± 0.083	4.90 ± 0.34	8.45	1.54 ± 0.29
H4 (1-21) R17A^*	0.11 ± 0.015	6.80 ± 0.34	61.82	1.17 ± 0.18	1.22 ± 0.26	3.11 ± 0.37	2.55	1.85 ± 0.44
H4 (1-21) R17K^*	0.48 ± 0.048	2.72 ± 0.26	5.67	3.87 ± 1.45	0.50 ± 0.048	3.26 ± 0.18	6.52	2.46 ± 0.56
H4 (1-21) R17MMA^*	0.13 ± 0.029	9.25 ± 0.79	71.15	1.3 ± 0.33	0.38 ± 0.040	7.85 ± 0.39	20.66	1.98 ± 0.40
H4 (1-21) R19A^*	0.49 ± 0.065	3.28 ± 0.41	6.69	3.86 ± 1.87	1.34 ± 0.29	4.25 ± 0.53	3.17	1.73 ± 0.37
H4 (1-21) R19K^*	0.30 ± 0.034	7.02 ± 0.57	23.4	4.90 ± 2.28	0.95 ± 0.15	4.53 ± 0.35	4.77	1.43 ± 0.21
H4 (1-21) R3K^*	nd^†	nd^†	nd^†	nd^†	nd^†	nd^†	nd^†	nd^†

^*

Twenty micromolar AdoMet used (20:1 molar ratio of AdoMet: [methyl-³H]-AdoMet).

^{^†}

Amount of product formation was too low to accurately measure kinetic parameters.