Fig. 2.

HSPGs are specifically required for dendrite development of C4da neurons. (A–F) DdaC in the Gal4^hh control (A–C) and Gal4^hh-driven ttv RNAi (D–F) at three developmental stages. (G and H) Quantification of terminal dendrite density (G) and terminal dendrite number (H) in hh domains. **P ≤ 0.01; ***P ≤ 0.001; Student’s t test. (I and J) DdaC in the control (tubP-Gal80^ts; Gal4^hh) (I) and an Sulf1-expressing animal (tubP-Gal80^ts; hh > Sulf1) in which Sulf1 expression was induced by temperature shift (TS) (J). Both genotypes were subjected to TS. (K and L) Quantification of dendrite density (K) and terminal dendrite density (L) in hh domains of Gal4^hh control, hh > Sulf1 (TS), and hh > Sulf1 (constant expression). ***P ≤ 0.001; ns, not significant; ANOVA and Tukey’s HSD test. For all quantifications, each circle represents a neuron. The numbers of neurons are indicated. Black bar, mean; red bars, SD. (Scale bars, 10 µm in A and D, 35 µm in B and E, and 50 µm in the rest of panels.)