Fig. 9.

DM NPF neurons are sufficient to induce preference in the two-choice arena. (A and B) Representative brains of single flies expressing CsChrimson in DM neurons only (A), or in DM plus other neurons (B) (example shows expression in two neurons projecting to the FSB, arrows). (C) Experimental data expressed as the mean ± SE of the proportion of flies on the active side over time. The yellow box indicates the period of activation (DM neurons only, n = 19; DM plus other neurons, n = 19). (D) ΔTime (%) (preference) of flies during the activation phase, indicating no significant difference between the preference of flies with CsChrimson expression in DM neurons only or in DM plus other neurons. Female flies were used in this experiment as stochastic labeling was sparser than in males (unpaired t test; P > 0.05) (frequency of activation, 40 Hz; 617-nm LED light intensity, 5 µW/mm²). (Magnification: 40×.)