(A) Injection of full-length mpv17 mRNA leads to rescue of the roy orbison mutant phenotype, as demonstrated by the appearance of iridophores both in the eye and throughout the body of the fish (roy n=0/20 fish with eye iridophores, roy+mRNA injection, n=14/20 fish with eye iridophores). (B) ATG morpholino knockdown of MPV17 in the AB fish leads to a strong phenocopy of the roy phenotype, with loss of eye and body iridophores (AB =71/79 fish with eye iridophores, AB+morpholino injection, n=8/67 fish with eye iridophores). (C) Quantification of eye iridophores after morpholino injection to mpv17 shows a significant increase in # of fish with absent iridophores (*, p<0.05, paired t-test)