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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Cancer Cell Microenviron. 2017 Jul 10;4(2):e1566. doi: 10.14800/ccm.1566

Figure 2. CAF potentiates HNSCC progression including Cell proliferation, migration, invasion, and chemotherapy resistance.

Figure 2

A. MTT assay showing a significant (p<0.05) induction of proliferation by CAF conditioned medium in HNSCC cell lines (P<0.05). B. Transwell co-culture of HNSCC cell lines with CAFs showed a significant increase in cell invasion of three HNSCC cell lines, OSC19, FADU, and UMSCC22A when compared to normal fibroblasts (P<0.05). C. HNSCC Cells line were co-cultured with/without CAF and migrating cells were counted. CAF induced significant migration (P<0.05). D. HNSCC cell line was grown to confluency in six well plates and wound scratch was made across the center of the wells. Cells were allowed to heal in the presence of conditioned medium from either of normal fibroblast (NF) or CAF. The distance between the two edges was measured at 0 time and 24 Hrs. Conditioned media from CAFs induced wound closure at a significantly faster rate (P<0.05) than cells incubated with CM from NF. E. HNSCC cell lines were incubated in the presence or absence of CM from NF or CAF in an increasing concentration of cisplatin. HNSCC cells incubated with CM from CAFs were more resistant than those incubated with CM from NF at higher concentration of cisplatin (P<0.05).