Skip to main content
. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Cancer Cell Microenviron. 2017 Jul 10;4(2):e1566. doi: 10.14800/ccm.1566

Figure 3. CAFs increase metastatic efficiency of HNSCC in mouse xenograft models.

Figure 3

A–B. Mice were injected with a suspension of OSC19, OSC19 and CAF (1:1) or OSC19 and CAF (1:5) in matrigel on the flank (A) or on the tongue (B) and maintained until the tumor growth reached 15mm as recommended by the institutional policy. Mice were sacrificed and adjoining lymph nodes and lungs were excised and processed for histological examination. Results indicated CAFs promoted metastatic efficiency (p<0.05) both in the flank model and the tongue model. (C) Immunohistochemical staining for LYVE1, a lymphangiogenic marker, was performed to measure the role of BDNF in potentiation of lymphangiogenesis in mice injected with OSC19, OSC19 and CAF transfected with vector or OSC19 and CAF transfected with BDNF construct. Overexpression of BDNF in CAFs significantly increased lymphangiogenic network as measured by LYVE1 intensity, p<0.05.