Figure 7. Antibodies to three cell surface expression markers increases phagocytosis and the potency of antibodies to induce phagocytosis is related to expression levels.

A.-C. Cells were labelled with 10 μg/mL of antibody, then AF488-conjugated secondary before analysing live cells by flow cytometry. Representative plots of three repeats. D.-F. Target cells were stained with TAMRA, and macrophages with CFSE then coincubated for 2 hours at 1:1 ratio with isotype or antibody. FcR Blocker is Fc receptor blocker, pre-incubated with U937 cells for 30 minutes prior to assay. Anti-CD47 and anti-CD19 at 2 µg/mL, anti-CD10 at 5 µg/mL. N = 3. * / ** / *** / **** p < 0.05 / 0.01 / 0.001 / 0.0001, compared with control, # as indicated, NS not significant.