Skip to main content
. 2017 May 5;8(37):60962–60974. doi: 10.18632/oncotarget.17631

Figure 2. CAT-1 promotes, and CAT-1 knockdown reduces, cell proliferation and increases colony formation.

Figure 2

(A) Cell proliferation of HepG2 transfected with pCAT-1, pcDNA, psiCAT-1, psi-NC, pCAT-1 with psiCAT-1 as detected by MTT assay. (B) CAT-1 expression analyzed by western blotting with β-actin as internal reference. (C, D) Cloning efficiency assessed by cell colony formation assay. (E) Cell proliferation of Hep3B transfected with pCAT-1, pcDNA, psiCAT-1, psi-NC, pCAT-1 with psiCAT-1 as-detected by MTT assay. (F) Cell proliferation of Huh7 transfected with pCAT-1, pcDNA, psiCAT-1, psi-NC, pCAT-1 with psiCAT-1 as-detected by MTT assay. Statistically significant differences are indicated: *p <0.05, **p<0.01, ***p<0.001, ****p<0.0001. Data represent mean ± SD. Experiments were performed in triplicate.