Skip to main content
. 2017 May 9;8(37):61001–61010. doi: 10.18632/oncotarget.17720

Figure 2. Tim-3 reverses ox-LDL-induced inhibition of HUVECs migration.

Figure 2

Wound-healing experiments were used to measure the vertical migration of HUVECs stimulated with ox-LDL (10 μg/mL) in the presence or absence of Tim-3 (1,000 ng/mL) or anti-Tim-3 mAb (10 μg/mL) after 48 hours. Representative images were obtained along the furrows after 48 hours of stimulation. The total cell numbers was counted after 48 hours of the respective treatment. The migration index was calculated by the following formula: Migration Index =Mtest/Ntest ÷ Mcon/Ncon × 100, where Mtest represents the number of migrated cells under different treatments, Ntest represents the total number of cells subjected to the respective treatments, Mcon represents the number of migrated cells under control treatment, Ncon represents the number of total cells under the corresponding control treatment. Data represent mean ± SEM. ***P < 0.001 compared with the control group.