Figure 6. AAV-Sirt6 delivery ameliorates bleomycin-induced pulmonary EMT.

(A) Immunofluorescent analysis of lung sections to determine the efficiency of Sirt6 delivery after mouse intratracheal injection of AAV-Sirt6 (2×10¹¹ vg) for 21 days. Scale bar, 50 μm; n=3 mice per group. (B-D) GFP^AAV and Sirt6^AAV mice were treated with 2 mg/kg bleomycin for 21 days. Lung sections were analyzed with IHC with antibodies against E-cadherin, α-SMA, and vimentin (magnification, 100×). n = 6 mice per group (B). Immunofluorescent staining was performed to measure proSPC and vimentin (C), and proSPC and α-SMA (D) expression in mouse lung. Scale bar, 50 μm; n = 3 mice per group.