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. 2017 May 9;8(37):61025–61035. doi: 10.18632/oncotarget.17731

Figure 4. Nur77 transcriptionally enhances A20 expression in monocytes and macrophages.

Figure 4

(A) qPCR assay (left) or Western blot analysis (right) of A20 expression in Nur77-overexpressing RAW264.7 cells. (B) qPCR assay (left) or Western blot analysis (right) of A20 expression in peritoneal macrophages from Nur77+/+ or Nur77-/- mice. (C, D) Western blot analysis of the indicated protein in Nur77-overexpressed RAW264.7 cells (C) or Nur77+/+ and Nur77−/− peritoneal macrophages (MΦ) (D) treated with vehicle or poly (I:C) (20 μg/ml). (E) Immunohistochemical staining of A20 in the liver tissues from of wild-type mice and Nur77-/- mice after poly (I:C)/D-GalN injection. (F) The luciferase activity of A20 promoter was measured in RAW264.7 cells transfected with the increasing amount of Nur77. (G) Binding of Nur77 to the A20 promoter by chromatin immunoprecipitation (ChIP) assay. ChIP assays were performed using anti-myc tag antibody in the THP-1 cells transfected Myc-Nur77 or vector. Statistical significance was determined using a two-tailed, unpaired Student's t-test. **P < 0.01.