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. 2017 May 12;8(37):61057–61071. doi: 10.18632/oncotarget.17841

Figure 2. BNTX induces TRAIL-induced apoptosis in AsPC-1 cells through caspase activation.

Figure 2

(A) Apoptosis signaling in AsPC-1 cells exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h analyzed by western blot analysis using the indicated antibodies. The arrows indicate the cleaved forms of caspase-8 and PARP. M: mitochondrial fraction; C: cytosol fraction. (B, C) AsPC-1 cells were pre-treated with caspase inhibitors z-IETD-fmk (20 μM) or z-DEVD-fmk (20 μM) for 1h, and then exposed to BNTX (2.5 μM) with or without TRAIL (25 ng/ml) for 24 h. Cell lysates were performed to measure apoptosis by caspase activity (B) and western blot analysis for caspase-3,-8 and PARP (C). Values are the mean ± SD from two independent experiments performed in duplicate. Asterisks indicate significant differences compared with the control (*** P < 0.001).