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. 2017 May 19;8(37):61133–61145. doi: 10.18632/oncotarget.18024

Figure 2. CNN1 is downregulated in the development of HGSC and in invasion progression of ovarian cancer cells.

Figure 2

(A) Quantitative RT-PCR and Western blot (B) analysis of CNN1 in primarily cultured fimbriae epithelial cells (FTEC, with the case number given), fimbria epithelial scrapings (FE, with case number given as prefix), as well as in immortalized (FE25) and transformed (FE-RAS) fimbria epithelial cell lines, two ovarian HGSC cell lines (Kuramochi, OVSAHO), and two common ovarian cancer cell lines (SKOV3, A1847) and their invasive subclone (I6 and I4). (C) Quantitative RT-PCR analysis of CNN1 in tissues of ovarian HGSC (OV-T, n = 6), fallopian tube HGSC (FT-T, n = 3), normal ovary (OV-N, n = 6), normal fallopian tube fimbria (FT-N, n = 5) as well as scrapings of normal fimbria epithelium (FE-N, n = 9). *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001. (D) Western blot analysis of CNN1 expression in clinical HGSC specimens derived from ovary (OV-T) and fallopian tube (FT-T). (E) Immunohistochemistry analysis of CNN1 expression in normal fallopian fimbriae and HGSC tissues.