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. 2017 May 24;8(37):61373–61384. doi: 10.18632/oncotarget.18139

Figure 3. Knock down of KIFC1 in HeLa cells by RNAi.

Figure 3

(A) WB analysis of knock down efficiencies using three different sets of siRNA. (B) Localization of KIFC1 in HeLa cells determined by immuno-florescent staining. (C) Quantification of WB band intensity from (A). The knock down efficiency is as follows: KD2 > KD1 > KD3. (D) Growth curve of cells with KIFC1 knock down. The negative control is transfected with siRNA targeting GFP. A=A450-A650, and A0 represents the A value at the time point of 0. (E-X) Dynamic behavior of cells with different levels of KIFC1 knock down was determined by wound-healing assay.