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. 2017 May 29;8(37):61425–61439. doi: 10.18632/oncotarget.18251

Figure 5. LAG-3 expression is upregulated and maintained by IL-12.

Figure 5

(A) LAG-3 expression on T cells cultured in anti-CD3 Ab-coated plate with anti-CD28 Ab in the presence or absence of IL-12 (50 ng/ml) for 3 days. Cells cultured in normal plate were used as a control. The right graphs summarize percentages of LAG-3+CD4+ or CD8+ T cells treated with or without IL-12 measured by flow cytometry. (B) LAG-3 mRNA expression in CD8+ T cells treated with or without IL-12 for 3 or 7 days. The results were expressed as log fold change of IL-12 treated vs untreated. (C) LAG-3 expression on T cells cultured in anti-CD3 Ab-coated plate with anti-CD28 Ab in the presence or absence of indicated cytokines for 3 and 7 days. (D) Co-expression of LAG-3 and TIM-3 on CD8+ T cells cultured in anti-CD3 Ab-coated plate with anti-CD28 Ab in the presence of escalated doses of IL-12 for indicated days. (E) CD8+ T cells were cultured in anti-CD3 Ab-coated plate with anti-CD28 Ab in the presence or absence of IL-12 for 5 days. A portion of cells was transferred into a normal plate for 7 days. Another portion of cells remained in the anti-CD3 Ab-coated plate for the same period. LAG-3 expression was measured on day 7. (F) Time-course measurement of LAG-3 expression on CD8+ T cells pre-treated with or without IL-12 after activation was withdrawn by transferring CD8+ T cells into normal plate. LAG-3 expression was measured on indicated days, n=3.