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. 2017 Jun 27;8(37):61528–61537. doi: 10.18632/oncotarget.18616

Figure 1. Increased SDC2 expression and kinase phosphorylation in melanoma cells under anchorage-independency.

Figure 1

(A) Expression of syndecan protein SDC1 and SDC2 under adhesion culture, A, or suspension culture, S, in different melanoma cells as analyzed by qPCR. Data were mean ±S.E. (n=3) **, p < 0.01. (B) SDC2 protein expression in A375 cells increased upon suspension as analyzed by western blot and flow cytometry. (C) Increased phosphorylation of PI3K, Akt, JNK, ERK, but not MEK, in suspended melanoma cells as examined by western blot.