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. 2017 Jun 27;8(37):61528–61537. doi: 10.18632/oncotarget.18616

Figure 5. SDC2 promoted chemosensitivity through PI3K and ERK pathways through glycosaminoglycan chains.

Figure 5

(A) SDC2 overexpression sensitized CPT-induced apoptosis in suspended melanoma. Suppression of SDC2 expression reduced CPT-induced apoptosis. ECL detection was film-exposed for 1 min (for SDC2 overexpression) or 3 min (for SDC2 suppression). (B) SDC1 overexpression or suppression did not affect the level of CPT-induced apoptosis in suspended melanoma. ECL detection was both film-exposed for 3 min. (C) Effect of LY294002 (PI3K inhibitor), U0126 (MEK/ERK inhibitor), SP600125 (JNK inhibitor), and triciribine (Akt inhibitor) on CPT-induced apoptosis in suspended melanoma. Decreased PARP cleavage was observed by treatment of PI3K inhibitor and ERK inhibitor at higher concentration. (D) Lower serum level protected suspended melanoma cells from CPT-induced apoptosis. (E) Treatment of glycosaminoglycan-synthesis inhibitor, xylopyranoside, suppressed CPT-induced apoptosis as seen by reduced PARP cleavage.