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. 2017 Jun 27;8(37):61674–61686. doi: 10.18632/oncotarget.18669

Figure 1. The expression of IGFBP-2 in pancreatic cancer cell lines and knockdown of IGFBP-2 inhibits pancreatic cancer cells migration and invasion.

Figure 1

(A) The expression of IGFBP-2 in Aspc-1, Bxpc-3, CFPAC, Panc-1, SW1990 and HPDE cells as shown by Western blot analysis. Higher level of IGFBP-2 expression was found in Bxpc-3 and CFPAC cells compared with HPDE. (B) The expression of IGFBP-2 after treatment with IGFBP-2 siRNA. (C) Migratory abilities of Bxpc-3 and CFPAC cells after IGFBP-2 knockdown were recorded for 48h using microscope. (D) Representative images of migration and invasion assays for Bxpc-3 and CFPAC cells after IGFBP-2 knockdown were visualized after 48h and 72h, respectively. The number of cells was counted (bottom panel) (Original mignification, 20×). (E) IGFBP2 mRNA levels were determined using qRT-PCR. The knockdown rate of sh-IGFBP2 was nearly 70%. (F) At day 35, all mice were killed and the metastatic nodules were evaluated. Data was presented as the means±SD of three independent experiments. ** compared with control, P<0.01. *** compared with control, P<0.001.