Figure 2. Knockdown of IGFBP-2 attenuates EMT of pancreatic cancer cells.

(A) Western blot showed that knockdown of IGFBP-2 resulted in enhanced expression of the E-cadherin epithelial marker and reduced expression of mesenchymal markers (N-cadherin and vimentin). (B) Single and merged images were taken to show immunofluorescence staining of E-cadherin (green) and vimtin (red) accompanied by the cell nucleus (blue) stained by DAPI. Silencing IGFBP-2 elevated the expression of E-cadherin and decreased the expression of vimentin (Original mignification, 20×). (C) The expression levels of E-cadherin and N-cadherin were detected by immunohistochemistry in paraffin-embedded tissue sections from the orthotopic pancreatic cancer models (Original mignification, 20×). The results represent means±SD of experiments performed in triplicate. ** compared with control, P<0.01.