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. 2017 Jun 27;8(37):61674–61686. doi: 10.18632/oncotarget.18669

Figure 5. Knockdown of IGFBP-2 inhibits proliferation and increases sensitivity to gemcitabine in vitro.

Figure 5

(A) The CCK-8 assay was performed to detect cell proliferation of 24, 48 and 72 h after cells infection. (B) Colony formation assays using the Bxpc-3 and CFPAC cell lines are shown for the NC and Lv-IGFBP2 groups. (C) The Bxpc-3 and CFPAC cells (NC and Lv-IGFBP2) were incubated with increasing doses of gemcitabine (0-10 μM and 0-100 μM, respectively). The sensitivity of pancreatic cancer cells to gemcitabine was significantly enhanced after IGFBP-2 knockdown. NC, infected with negative lentivirus. Data was reported as means ± SD for three independent experiments. ** compared with control, P<0.01. *** compared with control, P<0.001.