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. 2017 Jun 27;8(37):61742–61760. doi: 10.18632/oncotarget.18687

Figure 5. FAK knockdown inhibited metastasis of stathmin-overexpressing cells.

Figure 5

(A) Western blotting showed that FAK-specific siRNA decreased FAK expression. (B) The transwell assay revealed that the invasion number of the FAK-knockdown group was significantly decreased compared with that of the NC group. (C) The wound-healing assay was performed to detect the migratory potential of FAK-knockdown KYSE 170-STMN1 cells and showed that the motility of the FAK-knockdown group was decreased compared with that of the NC group. (D) The cell adhesion assay to investigate cell adhesion ability showed that the level of cell adhesion to FN was lower in the FAK-knockdown group than in the NC group. (E) Cells were incubated with 10 ug/ml antibody against integrinα5 45min before plating on FN. Histograms represent the absorbance (OD450) (***, P<0.001).