Figure 4. PAR-2 antagonist reversed the ambulatory pain, thermal and mechanical hyperalgesia induced by PC supernatant.

(A) Addition of PAR-2 antagonist FS-NH₂ (100 μM) into the PC supernatant (25μl) immediately before injection reduced ambulatory pain in rats at each time point during observation. (B) FS-NH₂ increased the PWL in rats received PC supernatants injection in response to thermal stimulus. (C) FS-NH₂ increased the PWT in rats received PC supernatants injection in response to mechanical stimulus. PC: supernatants cultured from pancreatic cancer tissues; FS: FSLLRY-NH₂, PAR-2 antagonist peptide; LR: LRGILS-NH_2, control peptide. *P<0.05, **P<0.01 compared with control peptide group; n=6 in each group.