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. 2017 Jun 27;8(37):61810–61823. doi: 10.18632/oncotarget.18696

Figure 5. PC supernatant induced DRG neurons releasing of SP and CGRP in a protease-dependent way.

Figure 5

After incubation for 2h, the releasing of SP (A) and CGRP (B) form cultured DRG neurons were higher in PC supernatant group than that in NC supernatant group. Similarly, the mRNA levels for SP (C) and CGRP (D) were significantly increased in DRG neurons treated with PC supernatants. Co-incubation with protease inhibitor FUT-175 (50μg/ml) and PC supernatant reduced this neuropeptide release effect in cultured DRG neurons. Ctr: unstimulated DRG. PC: supernatants cultured from pancreatic cancer tissues. NC: supernatants cultured from normal pancreatic tissues. F: a broad-spectrum serine protease inhibitor, FUT-175.*P<0.05 n=6 in each group.