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. 2017 Jun 28;8(37):61901–61916. doi: 10.18632/oncotarget.18717

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Copyright: © 2017 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) MTT assay demonstrated the restoration of sensitivity to pemetrexed in a pemetrexed-resistant cell line (PC-9R-A) after siRNA-mediated astrocyte-elevated gene-1 (AEG-1) knockdown (PC-9R-A+AEG-1 siRNA transfection). (B) Upregulation of relative AEG-1 and thymidylate synthase (TS) mRNA levels in a pemetrexed-resistant cell line (PC-9R-A) and their sensitivity to siRNA-mediated AEG-1 knockdown (PC-9R-A+AEG-1 siRNA transfection) was revealed by RT-PCR. *: compare with PC-9, p < 0.05; **: compare with PC-9R-A, p < 0.05. (C) Effect of transfection with AEG-1 siRNA on AEG-1 and TS protein (left panel) and gene expression (right panel) levels was detected using western blotting and RT-PCR. *: compare with PC-9, p < 0.05; **: compare with PC-9R-A, p < 0.05. (D) Flow cytometry dot plots and quantitative analysis showed increased apoptosis rate in PC-9R-A cells after AEG-1 knockdown. *p < 0.05.

(A) MTT assay demonstrated the restoration of sensitivity to pemetrexed in a pemetrexed-resistant cell line (PC-9R-A) after siRNA-mediated astrocyte-elevated gene-1 (AEG-1) knockdown (PC-9R-A+AEG-1 siRNA transfection). (B) Upregulation of relative AEG-1 and thymidylate synthase (TS) mRNA levels in a pemetrexed-resistant cell line (PC-9R-A) and their sensitivity to siRNA-mediated AEG-1 knockdown (PC-9R-A+AEG-1 siRNA transfection) was revealed by RT-PCR. *: compare with PC-9, p < 0.05; **: compare with PC-9R-A, p < 0.05. (C) Effect of transfection with AEG-1 siRNA on AEG-1 and TS protein (left panel) and gene expression (right panel) levels was detected using western blotting and RT-PCR. *: compare with PC-9, p < 0.05; **: compare with PC-9R-A, p < 0.05. (D) Flow cytometry dot plots and quantitative analysis showed increased apoptosis rate in PC-9R-A cells after AEG-1 knockdown. *p < 0.05.