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. 2017 Jun 28;8(37):61969–61981. doi: 10.18632/oncotarget.18755

Figure 5. p38MAPK signaling contributes to expression of Fibronectin in response to cytokines and tumor-fibroblast interactions.

Figure 5

(A) Immunoblotting of Fibronectin and tubulin, a loading control, in whole-cell lysates from empty-vector control (EGFP) and dn-p38 MDA-MB-231 tumor cells treated with 2 ng/mL TGF-β1 or 10 ng/mL TNF or their combination for the indicated times. (B) Immunoblot analysis of Fibronectin and α-catenin, a loading control, in lysates of tumor cells treated with SB202190, a p38 inhibitor, and 2 ng/mL TGF-β1 for 24 hours. (C) Immunoblots of Fibronectin, p38, p-Smad2, p-RELA and GAPDH, a loading control, in lysates of MDA-MB-231 cells transfected with Scramble-control or siRNA to p38-alpha and then treated with 2 ng/mL TGF-β1 or 10 ng/mL TNF or their combination for the indicated times. (D-E) Immunoblotting of Fibronectin, phospho-HSP27 and tubulin, a loading control, in lysates from co-cultures (T+F) of MDA-MB-231 and A549 cancer cells (T) with 208F and WI-38 fibroblasts (F) for 72 hours. (F) Immunoblots of Fibronectin and GAPDH, a loading control, in lysates from co-cultures (T+F) of MDA-MB-231 EGFP or dn-p38 cells (T) and 208F fibroblasts (F) incubated for 48 hours.