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. 2017 Jul 7;8(37):62081–62098. doi: 10.18632/oncotarget.19060

Figure 10. Effect of BIX01294 on the tumor growth curve of SiHa cells.

Figure 10

SiHa cell lines were seeded in the right hind limb of SCID mice. (A) The mice were divided randomly into three groups. In the first group, vehicle was injected intraperitoneally (normal saline, 100 μL, twice a week). For the second group, mice were injected intraperitoneally with BIX01294 at 5 mg/kg (mouse body weight; dissolved in normal saline, 100 μL, twice a week). Finally, in the third group mice were injected intraperitoneally with BIX01294 at 10 mg/kg (mouse body weight; dissolved in normal saline, 100 μL, twice a week). In the BIX01294 10mg/kg group, tumor growth was inhibited significantly 29 d after inoculation. In the BIX01294 5mg/kg group, tumor growth did not differ significantly from the control group (n = 10 in each group. *p < 0.05; **p < 0.01; ANOVA with post hoc Tukey’s test). Upward arrow ↑ (in black): cancer cell inoculation. Downward arrow ↓ (in green): vehicle or BIX01294 injection. (B) Tumors from vehicle and BIX01294 (10 mg/kg) treatment groups were harvested at the time of sacrifice (day 39 after inoculation) and fixed in 10% neutral buffered formalin and processed for proliferating cell nuclear antigen (PCNA). Representative images of PCNA staining are shown in the upper panel (400x). Quantification of the positive rate is shown in the lower panel. n = 20 in each group. ****p < 0.0001. (C) Representative images of CD31 staining in tumor xenografts are shown in the upper panel (400x). Microvessel density (MVD) counts are shown in the lower panel. n = 20 in each group. ****p < 0.0001. (D) Representative images of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) are shown in the upper panel (400x). TUNEL counts are shown in the lower panel. n = 20 in each group. p > 0.05. Data are presented as mean ± SD. HPF: high power field.