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. 2017 Jul 7;8(37):62081–62098. doi: 10.18632/oncotarget.19060

Figure 6. Angiogenic factors’ gene expression as determined by G9a gene silencing, pharmacologic inhibition of G9a, and G9a overexpression.

Figure 6

Cervical cancer cells (SiHa, HeLa and CaSki) were treated in one of the following ways: with control siRNA or G9a siRNA for 24 hrs; with BIX01294 (5 μM) or vehicle for 24 hrs; or with transient transfection with control plasmid (mock) or G9a over-expression plasmid (G9a-oe) for 48 hrs. The angiogenin, interleukin-8 (il-8) or cxcl16 mRNA expression was determined by real-time quantitative RT-PCR. Data presented here are the relative percentages of induction. Data are compared between the indicated groups. *p < 0.05, n = 3.