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. 2017 Jul 10;8(37):62143–62153. doi: 10.18632/oncotarget.19121

Figure 5. miR-217 directly regulates AEG-1 and PD-L1 in Hep2 cells.

Figure 5

(A) Prediction of miR-217 binding sites on AEG-1 and PD-L1. (B) Prediction of miR-217 mutant binding sites on AEG-1 and PD-L1. (C) Luciferase activity in Hep2 cells infected with miR-217 or control after transfection with the indicated 3′-UTR-driven reporter plasmid or mutant 3′-UTR-driven reporter plasmid (N=3). (D) Real-time PCR results for endogenous AEG-1 and PD-L1 infected with miR-217 or NC (N=3). (E) Western blot results for endogenous AEG-1 and PD-L1 infected with miR-217 or NC.