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(A) qRT-PCR assay to measure PD-L1 mRNA levels when AEG-1 was overexpressed (N=3). (B) Western blot assay to measure PD-L1 protein levels when AEG-1 was overexpressed . (C) Luciferase assay showing activity for co-transfection of AEG-1 and promoter vector in Hep2 cells (N=3).
