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. 2017 Jul 10;8(37):62217–62230. doi: 10.18632/oncotarget.19216

Figure 5. CTCF regulates the majority of the analyzed metastasis-associated genes in ovarian cancer.

Figure 5

(A) PCR-array analysis of 89 genes associated with tumor metastasis was performed to compare the gene expression levels between vector- and shCTCF1-transfected SKOV3 and A2780 cells. Genes whose expression levels changed more than 2-fold are shown. (B) The fold-change of 89 genes in (A) is expressed as a heat map. (C) The expression of CTBP1, SERPINE1 and SRC was examined by Western blot analysis in vector- and shCTCF1-transfected SKOV3 and A2780 cells and in xenografted tumors derived from the metastasis mouse models. Representative data from 3 independent experiments are shown. (D) Peak regions of CTCF binding sites for the gene of SRC, CTBP1 and SERPINE1 are analyzed by Chip-seq in SKOV3 cells. (E) CTCF binding sites analyzed by Chip-seq for the gene of SRC, CTBP1 and SERPINE1 in SKOV3 cells are further confirmed by Chip-PCR, purified input DNA and normal mouse IgG pulldowns are used as control.