Video 2. Confocal projection of an Arabidopsis seedling 70 hr after treatment with NPA showing view of the vegetative meristem from above with channels for PIN1-CFP (blue), REV-2x YPet (red) and KAN1-2xGFP (green) alternating (also shown as a snapshot in Figure 11C).

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DOI: 10.7554/eLife.27421.019

Note the absence of contiguous KAN1 expression in between the meristem and older leaves potentially due to auxin build up in the absence of its regular transport.