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. 2017 Sep 12;6:e28409. doi: 10.7554/eLife.28409

Figure 7. The Syt1-SNARE interaction surface (Zhou et al., 2015) reveals that residues S332 (S279 in mammalian Syt1), R334 (R281), Y391 (Y338), E348 (E295), and A455 (A402) are positioned at the Syt1-SNARE interface.

Figure 7.

All five residues were identified as suppressors in our genetic screen. (A,B) Two perpendicular views of the Syt1-SNARE complex are shown. The residues listed above are shown in yellow. Orange – synaptobrevin; cyan – syntaxin; red – SN1 domain of SNAP25; silver – SN2 domain of SNAP25. (C,D) Magnified views of opposite surfaces of the Syt1-SNARE interface are shown. Note that the Syt1-SNARE interaction is supported by a salt bridge between R334 (R281) of Syt1 and E55 of SNAP25. In addition, S332 (S279) forms hydrophobic interactions with V48 of SNAP25.