Semen analysis cohort inclusion criteria. Sample selection for the men with semen analysis. Matched fertile control subjects were selected for the final samples: 12,889 for the first-degree relative (FDR) analysis and 8,032 for the second-degree relative (SDR) analysis. Total number of FDRs and SDRs for men with semen analysis and their matched control subjects by relationship type. Total numbers of first- and second-degree relatives equal 130,689 and 247,204, respectively.