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. Author manuscript; available in PMC: 2018 Feb 10.
Published in final edited form as: J Control Release. 2017 Jan 6;247:167–174. doi: 10.1016/j.jconrel.2017.01.006

Fig. 6. Skin accumulation of DiR labeled liposomes loaded with 3kDa dextran-IRDye 680.

Fig. 6

A) Dual-labeled liposomes were washed by dialysis, pelleted with ultracentrifuge, spotted on a nitrocellulose membrane and imaged with Li-COR odyssey (800nm-DiR; 700nm-IRdye680). There was no free dye in the solution (supernatant) after centrifugation; B) circulation of liposomes in mice after systemic injection. Both DiR and dextran showed long half-life, albeit dextran had a shorter half-life, likely do to partial release from liposomes in vivo and faster clearance than liposomes (n=2); C–D) Accumulation of fluorescence in the skin and small intestine (scanned with Li-COR after sacrificing the mice 200 h post-injection). Colocalization of DiR and dextran-IRDye 680 in the skin and intestine suggests that liposomes arrive with the payload to the tissue. Control non-injected mice show some signal in the 700nm channel (autofluorescence).