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Properties of fluorophores used in the study and the optical setup
| Spectral channels (LED) | 760nm | 670nm | ||
|---|---|---|---|---|
| Filter settings | Ex: 785nm short pass/Em: 785nm long pass |
Ex: 692nm short pass/Em: 694nm long pass |
||
| Fluorophore | DiR | IRDye800 | IRDye680 | |
| Peak Excitation | 750nm | 778nm | 680nm | |
| Peak Emission | 773nm | 774nm | 694nm | |
| Extinction Coefficient (ε; M−1cm−1) | 199,000 | 300,000 | 170,000 | |
