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. 2017 Sep 18;127(10):3845–3860. doi: 10.1172/JCI92067

Figure 5. NFATc1 mediates the oxidative conversion of myofibers by Gα13 ablation.

Figure 5

(A) Immunostain images for myosin heavy chains and histochemical assays for SDH activity of tibialis anterior muscles 14 days after electroporation-mediated gene delivery. Each mouse of the indicated genotype received a control shRNA vector in 1 limb and a plasmid expressing shNFATc1 in the contralateral limb. Type 1 and 2a myofibers and those with high SDH activity were quantified. Scale bars: 200 μm. (B) Respiration assay and immunoblots. Basal OCRs were determined in C2C12 myotubes of the indicated genotypes, which were prepared by CRISPR-mediated gene editing (n = 3 each). Blots were obtained from samples run on parallel gels. DKO, double-KO. All data represent the mean ± SEM. *P < 0.05 and **P < 0.01, by Student’s t test.