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. 2017 Sep 5;127(10):3657–3674. doi: 10.1172/JCI93041

Figure 4. Bone marrow cells from Dnmt3a+/– mice display myeloid skewing and a competitive advantage that is time dependent.

Figure 4

(A and B) Flow cytometric evaluation of lineage markers from the bone marrow cells of unmanipulated mice harvested at the indicated ages, designated in months (n = 1 per genotype per time point). (A) Dnmt3a+/+ mice. (B) Dnmt3a+/– mice. (C–G) Bone marrow from 6-week-old Dnmt3a+/+ or Dnmt3a+/– mice (Ly5.2) was mixed 50:50 with WT competitor marrow (Ly5.1x5.2) and transplanted into lethally irradiated WT mice (Ly5.1). n = 13 Dnmt3a+/+; n = 10 Dnmt3a+/–. (C) Peripheral blood chimerism at 4 months, 6 months, and 1 year after transplant. P < 0.01, 2-sample, 2-tailed t test. (D–G) Percentage of Ly5.2+ cells (i.e., experimental cells, either Dnmt3a+/+ or Dnmt3a+/–) in the indicated lineage or progenitor populations at the 1-year time point. *P < 0.05, 1-sample, 2-tailed t test vs. 50% corrected for multiple testing by Bonferroni’s method. (D) Peripheral blood–derived cells. (E) Spleen-derived cells. (F and G) Bone marrow–derived cells.