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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: J Am Chem Soc. 2017 Jun 29;139(27):9203–9212. doi: 10.1021/jacs.7b02716

Figure 1.

Figure 1

The sur biosynthetic gene cluster and associated reporter constructs. (A) The sur cluster; surA–surD encode nonribosomal peptide synthetases, surE encodes an α,β-hydrolase, and surR encodes a GntR-type transcriptional regulator. The black arrow represents the promoter. (B) Genetic constructs utilized in this study. Triple eGFP driven by Psur inserted at a neutral site (attB::Psur-eGFPx3) or in place of surE (surE::eGFPx3). (C) Reporter assays measuring signal amplification with one, two, or three copies of eGFP driven by PermE.