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. Author manuscript; available in PMC: 2018 Sep 28.
Published in final edited form as: J Am Chem Soc. 2017 Mar 28;139(14):5051–5060. doi: 10.1021/jacs.6b11323

Figure 3. Secondary-structure of consensus-HD determined by NMR spectroscopy.

Figure 3

A) Plot of chemical shift-based secondary structure predictions by TALOS+29 B) 1HNi- 1HNi+1 and C) 1Hαi- 1HNi+3 NOEs. Strong NOEs were given value of one. Weak but detectable NOEs were given a value of 0.5. Positions with no bar showed no detectable NOE. Helix boundaries (top) are based on the engrailed-HD structure. Sequence positions are as in alignment in Figure 1B where position 1 corresponds to the N-terminal D in engrailed-HD and position 2 corresponds to the N-terminal R in consensus-HD. Conditions: 25 mM sodium phosphate, 150 mM NaCl, pH 7, 20 °C.