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. Author manuscript; available in PMC: 2018 Sep 28.
Published in final edited form as: J Am Chem Soc. 2017 Mar 28;139(14):5051–5060. doi: 10.1021/jacs.6b11323

Figure 5. Stability and folding kinetics of consensus-HD compared to engrailed-HD.

Figure 5

A) Guanidine-induced unfolding of consensus-HD (black) and engrailed-HD (red) at 20 °C. B) Guanidine dependence of the refolding and unfolding rate constant of consensus-HD at 20 °C. C) Guanidine dependence of the refolding and unfolding rate constants of consensus-HD (black) and engrailed-HD (red) at 10 °C. Solid lines are obtained from fitting a kinetic two-state model (equation 3) to the data. Conditions: 25 mM sodium phosphate, 150 mM NaCl, pH 7, temperature noted.