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. Author manuscript; available in PMC: 2018 Sep 28.
Published in final edited form as: J Am Chem Soc. 2017 Mar 28;139(14):5051–5060. doi: 10.1021/jacs.6b11323

Figure 7. Isothermal titration calorimetry of consensus- and engrailed-HDs binding to DNA.

Figure 7

A) Differential power and B) integrated heat peaks resulting from titration of the engrailed-HD into DNA. C) Differential power and D) integrated heat peaks resulting from titration of the consensus-HD into DNA. The black lines show a least-squares fit using a single-site model. Conditions: 25 mM HEPES, 250 mM KCl pH 7.5, 25 °C.