Table 3.
Real time RTPCR and Corticosterone results from Experiment 2
| Homecage | Homecage-Swim (1 day) | Homecage-Swim (5 days) | Restraint-Swim (1 day) | Restraint-Swim (5 days) | Statistical analyses | |
|---|---|---|---|---|---|---|
| PVN | ||||||
| β-actin | 99.8 ± 17.4 | 101.4 ± 25.1 | 93.3 ± 22.3 | 124.0 ± 19.9 | 120.8 ± 23.9 | F(4,35) = 0.38, p > 0.05 |
| TNF-α | 112.3 ± 19.8 | 118.7 ± 23.1 | 64.6 ± 33.3 | 124.3 ± 34.3 | 67.6 ± 15.9 | F(4,34) = 1.57, p > 0.05 |
| IL-1β | 114.2 ± 18.3a | 118.1 ± 18.0a | 205.1 ± 19.0b | 230.7 ± 41.9b | 162.3 ± 22.3ab | F(4,34) = 4.53, p < 0.01 |
| IL-6 | 100.4 ± 40.0 | 94.9 ± 18.9 | 76.3 ± 16.4 | 152.6 ± 61.4 | 102.5 ± 33.0 | F(4,31) = 0.80, p > 0.05 |
| c-Fos | 118.3 ± 27.3a | 557.8 ± 92.9bc | 388.3 ± 60.0b | 675.4 ± 68.3c | 470.5 ± 117.1bc | F(4,32) = 7.18, p < 0.001 |
| CD14 | 119.5 ± 23.4 | 132.0 ± 42.0 | 113.6 ± 15.4 | 171.1 ± 76.9 | 110.1 ± 21.6 | F(4,32) = 0.33, p > 0.05 |
| CD200 | 142.8 ± 42.4 | 139.9 ± 38.7 | 62.5 ± 32.1 | 152.7 ± 41.3 | 147.1 ± 38.2 | F(4,34) = 0.86, p > 0.05 |
| CD200R | 115.1 ± 21.9 | 114.7 ± 19.2 | 89.3 ± 14.4 | 178.3 ± 71.2 | 90.2 ± 18.1 | F(4,35) = 0.31, p > 0.05 |
| GFAP | 117.1 ± 21.5 | 88.1 ± 14.2 | 87.3 ± 13.5 | 86.4 ± 14.3 | 83.5 ± 15.5 | F(4,34) = 0.76, p > 0.05 |
| HPC | ||||||
| β-actin | 102.1 ± 7.2 | 93.9 ± 16.2 | 89.1 ± 9.8 | 85.9 ± 7.9 | 101.4 ± 7.7 | F(4,36) = 0.51, p > 0.05 |
| TNF-α | 102.4 ± 7.6 | 149.3 ± 34.0 | 123.3 ± 9.6 | 119.8 ± 13.9 | 103.9 ± 9.9 | F(4,35) = 1.12, p > 0.05 |
| IL-1β | 105.1 ± 11.9 | 97.0 ± 12.9 | 126.0 ± 19.9 | 122.2 ± 11.8 | 74.9 ± 7.7 | F(4,36) = 2.33, p > 0.05 |
| IL-6 | 101.7 ± 6.7a | 115.8 ± 21.0a | 179.2 ± 20.9ab | 219.3 ± 38.8b | 240.1 ± 27.4b | F(4,36) = 4.93, p < 0.01 |
| c-Fos | 103.9 ± 9.9a | 605.6 ± 74.2b | 277.8 ± 14.5c | 816.6 ± 100.3d | 270.1 ± 17.9c | F(4,36) = 27.40, p < 0.001 |
| CD14 | 103.6 ± 9.5 | 146.8 ± 40.0 | 137.0 ± 20.8 | 151.2 ± 22.4 | 118.4 ± 9.7 | F(4,36) = 0.75, p > 0.05 |
| CD200 | 101.6 ± 6.2 | 93.5 ± 9.5 | 99.8 ± 9.6 | 111.3 ± 9.1 | 100.6 ± 9.5 | F(4,36) = 0.51, p > 0.05 |
| CD200R | 101.3 ± 6.2 | 113.6 ± 9.5 | 86.1 ± 9.6 | 120.2 ± 9.1 | 88.9 ± 9.5 | F(4,35) = 1.48, p > 0.05 |
| GFAP | 102.6 ± 8.4a | 89.9 ± 8.3a | 86.3 ± 8.5a | 133.1 ± 18.9b | 96.5 ± 4.6a | F(4,36) = 2.95, p < 0.05 |
| PFC | ||||||
| β-actin | 134.4 ± 22.0 | 159.8 ± 27.2 | 76.7 ± 18.9 | 149.7 ± 15.6 | 127.6 ± 13.6 | F(4,35) = 1.99, p > 0.05 |
| TNF-α | 108.2 ± 14.9a | 80.3 ± 8.8b | 81.8 ± 11.1ab | 56.9 ± 6.1bc | 46.8 ± 4.7c | F(4,34) = 5.76, p < 0.01 |
| IL-1β | 113.8 ± 22.2 | 110.6 ± 16.1 | 126.1 ± 16.7 | 118.1 ± 19.9 | 83.9 ± 11.4 | F(4,33) = 0.64, p > 0.05 |
| IL-6 | 108.4 ± 16.0 | 152.1 ± 20.7 | 189.0 ± 50.5 | 222.8 ± 40.5 | 221.5 ± 35.3 | F(4,33) = 2.04, p > 0.05 |
| c-Fos | 105.2 ± 11.5a | 1347.9 ± 142.4b | 576.9 ± 65.9c | 1697.9 ± 157.8d | 614.3 ± 90.4c | F(4,33) = 43.59, p < 0.001 |
| CD14 | 102.3 ± 7.8a | 106.4 ± 13.9a | 160.9 ± 23.0b | 85.2 ± 7.0a | 80.2 ± 8.1a | F(4,34) = 4.81, p < 0.01 |
| CD200 | 106.0 ± 9.9 | 125.9 ± 12.6 | 101.9 ± 16.9 | 122.7 ± 8.5 | 121.1 ± 10.6 | F(4,33) = 0.84, p > 0.05 |
| CD200R | 106.7 ± 12.7 | 110.8 ± 17.2 | 173.6 ± 60.4 | 106.4 ± 10.0 | 94.3 ± 60.4 | F(4,35) = 1.32, p > 0.05 |
| GFAP | 104.2 ± 9.7 | 115.1 ± 23.7 | 131.6 ± 34.5 | 88.5 ± 13.4 | 130.0 ± 18.2 | F(4,33) = 0.87, p > 0.05 |
| Corticosterone (μg/dl) | ||||||
| CORT | 1.18 ± 0.47a | 42.25 ± 8.68b | 49.53 ± 8.02b | 42.79 ± 7.38b | 48.29 ± 6.16b | F(4,35) = 62.45, p < 0.001 |
Note. Means and SEM for Experiment 2. Relative gene expression data in the paraventricular nucleus of the hypothalamus (PVN), prefrontal cortex (PFC), and hippocampus (HPC) were adjusted to β-actin as a housekeeper and expressed as percent change from the control group (homecage). Corticosterone data is in μg/dl. Bold text indicates a significant effect; data points marked with differing letters are significantly different from one another (p < 0.05).